OsNAC5 در گیاهان تراریخته موجب افزایش عملکرد می شود.

OsNAC5 overexpression enlarges root diameter in rice plants leading to enhanced drought tolerance and increased grain yield in the field

این مقاله در سال 2013 در مجله Plant Biotechnology Journal    به  این ادرس در دسترس است. این مقاله دارای 300، citation می باشد.

خشکی از مهم ترین چالش های پیش روی تولید جهانی محصولات کشاورزی است. در مطالعه حاضر، ارزیابی میدانی افزایش عملکرد برنج تراریخته با بیان بالای OsNAC5 در شرایط نرمال با پروموتور های اختصاصی ریشه(RCc3) و یا پروموتورهای (GOS2) ارزیابی های مزرعه ای در سه فصل رشد در رابطه با عملکرد دانه RCc3:OsNAC5 and GOS2: OsNAC5 نشان داد که 9 تا 23 درصد و 9 تا 26 درصد به ترتیب، برای هرکدام در شرابط نرمال افزایش عملکرد صورت گرفته است. در شرایط خشکی گیاهان RCc3:OsNAC5 افزایش عملکرد چشم گیری به میزان 22 تا 63 درصد در حالیکه GOS2:OsNAC5  عملکرد تفاوتی با عملکرد نمونه غیر تراریخته در شرایط کنترل نداشت.

Summary

Drought conditions are among the most serious challenges to crop production worldwide. Here, we report the results of field evaluations of transgenic rice plants overexpressing OsNAC5, under the control of either the root-specific (RCc3) or constitutive (GOS2) promoters. Field evaluations over three growing seasons revealed that the grain yield of the RCc3:OsNAC5 and GOS2:
OsNAC5 plants were increased by 9%–23% and 9%–26% under normal conditions, respectively. Under drought conditions, however, RCc3:OsNAC5 plants showed a significantly higher grain yield of 22%–63%, whilst the GOS2:OsNAC5 plants showed a reduced or similar yield to the nontransgenic (NT) controls. Both the RCc3:OsNAC5 and GOS2:OsNAC5 plants were found to have larger roots due to an enlarged stele and aerenchyma at flowering stage. Cell numbers per cortex layer and stele of developing roots were higher in both transgenic plants than NT controls, contributing to the increase in root diameter. The root diameter was enlarged to a greater extent in the RCc3:OsNAC5, suggesting the importance of this phenotype for enhanced drought tolerance. Microarray experiments identified 25 up-regulated genes by more than three-fold (P < 0.01) in the roots of both transgenic lines. Also identified were 19 and 18 up-regulated genes that are specific to the RCc3:OsNAC5 and OS2:OsNAC5 roots, respectively. Of the genes specifically up-regulated in the RCc3:OsNAC5 roots, GLP, PDX, MERI5 and O-methyltransferase were implicated in root growth and development. Our present findings demonstrate that the root-specific overexpression of OsNAC5 enlarges roots significantly and thereby enhances drought tolerance and grain yield under field conditions

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